Manual Processing Sets

Cord Blood Processing, Washing and Freezing Bag Sets for the Cryopreservation of Blood and Cellular Components

Freezing Bag (48/Box)

Transfer/Freezing Bag Set

Application

Designed for processing and freezing cord blood stem cells by the method developed at the New York Blood Center. 1, 2* Each sterile set consists of two transfer bags and a two-compartment, three-dimensional 25 mL freezing bag joined by integral tubing and two connector lines (one spike and one luer).

 

Description

  • Two transfer bags, 200 and 150 mL. The 200 mL transfer bag concentrates primary stem cell-rich plasma while the 150 mL transfer bag receives the supernatant plasma from the concentrated stem cells.1, 2* 
  • Freezing bag is compatible with liquid nitrogen (LN2) storage. The three-dimensional bag design ensures a homogenous, controlled freezing rate for maximum cell viability.
  • Freezing bag is uniquely compatible with the use of DMSO/Dextran and liquid nitrogen storage in The BioArchive® System.
  • Freezing bag’s two separable compartments allow for a 4:1 division of contents, allowing a consistent volume aliquot for ex vivo cell expansion.
  • Freezing bag is compliant with AABB Standard for integral segments cryopreserved with product, enabling confirmatory testing prior to transfusion.
  • Addition of cryopreservative solution can be accomplished by either gravity feed or syringe pump.

 

 

References

**SOP available at the EMMES website: http://spitfire.emmes.com/study/cord/

1 Rubinstein, P.; Dobrila, L.; Rosenfield, R.E.; Adamson, J.W.; Migliaccio, G.; Migliaccio, A.R.; Taylor, P.E.; Stevens, C.E. Processing and Cryopreservation of Placental/Umbilical Cord Blood for Unrelated Bone Marrow Reconstitution. Proc Natl Acad Sci USA. 1995, Oct 24;92(22):10119-22.

2 Fraser, J.K.; Cairo, M.S.; Wagner, E.L.; McCurdy, P.R.; Baxter-Lowe, L.A.; Carter, S.L.; Kernan, N.A.; Lill, M.C.; Slone, V.; Wagner, J.E .; Wallas, C.H.; Kurtzberg, J. Cord Blood Transplantation Study (COBLT): Cord Blood Bank Standard Operating Procedures. Journal of Hematotherapy. 1998, 7(6):521-61.

Information and photo courtesy of Pall Corp.

Freezing Bag

Application

For the long-term storage of 25 mL cryopreserved concentrated blood components.

 

Description

Each sterile set consists of a two-compartment, three-dimensional 25 mL freezing bag and a tubing lead terminating in a spike. The freezing bag has been designed to assure a homogenous, controlled freezing rate for maximum cell viability. The design simultaneously reduces the possibility of bag breakage from thermal, dynamic or expansion forces.

  • Freezing bag is compliant with AABB Standard for integral segments cryopreserved with product, enabling additional testing prior to transfusion.
  • Two separable compartments allow for a 4:1 division of contents, allowing a consistent volume aliquot for ex vivo cell expansion.
  • Compatible with liquid nitrogen (LN2) storage. Three-dimensional bag design ensures a homogenous, controlled freezing rate for maximum cell viability.
  • Freezing bag is uniquely compatible with DMSO/Dextran and liquid nitrogen storage in ThermoGenesis’ BioArchive® System
  • Compatible with the Cell Wash/Infusion Bag Set (PN 791-03).
  • Bag sold separately for connection to other processing sets.
  • Tubing is RF or heat sealable.

 

Note:

Freezing bags and bag sets that are manufactured by Pall Corp. are sold by ThermoGenesis Corp. in Asia, Latin America, Middle East, India and Africa and by Pall Corp. in North America and Europe. Freezing bags and bag sets for Japan are manufactured and distributed by Nipro Corporation.

Information and photo courtesy of Pall Corp.

 

Cell Wash/Infusion Bag Set

Application

For the thaw/washing and subsequent infusion of previously frozen cord blood stem cells as developed by the New York Blood Center.

 

Description

This two-bag set is designed to achieve a slow asymptotic reduction in osmotic pressure of the cryo-protected white cells to the appropriate level for transfusion. Set reduces the DMSO content of the final stem cell transfusion to less than one gram. Each sterile set consists of two transfer bags joined by integral tubing, and three connector lines (two spikes and one luer).

Designed specifically for use with the freezing bag of the Transfer/Freezing Bag Set (PN 791-02) and the Freezing Bag (PN 791-05). Components include:

  • Two 250 mL capacity transfer bags. One 250 mL transfer bag is used for thawing the frozen unit with a Dextran/albumin solution. The second 250 mL transfer bag receives supernatant from the concentrated stem cells.1,2*
  • Two multiple-use sampling sites.

 

References

*SOP available at the EMMES website: http://spitfire.emmes.com/study/cord/

1 Rubinstein, P.; Dobrila, L.; Rosenfield, R.E.; Adamson, J.W.; Migliaccio, G.; Migliaccio, A.R.; Taylor, P.E.; Stevens, C.E. Processing and Cryopreservation of Placental/Umbilical Cord Blood for Unrelated Bone Marrow Reconstitution. Proc Natl Acad Sci USA. 1995, Oct 24;92(22):10119-22.

2 Fraser, J.K.; Cairo, M.S.; Wagner, E.L.; McCurdy, P.R.; Baxter-Lowe, L.A.; Carter, S.L.; Kernan, N.A.; Lill, M.C.; Slone, V.; Wagner, J.E .; Wallas, C.H.; Kurtzberg, J. Cord Blood Transplantation Study (COBLT): Cord Blood Bank Standard Operating Procedures. Journal of Hematotherapy. 1998, 7(6):521-61.

Information and photo courtesy of Pall Corp.

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